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osteoblast data  (Oxford Instruments)


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    Structured Review

    Oxford Instruments osteoblast data
    Schematic of <t>osteoblast</t> morphology and behavior during bone nodule formation. Osteoblasts had a crowded cuboidal morphology in the early stages of bone formation; some were embedded in the bone matrix and became osteocytes, and the remaining osteoblasts acquired a flattened BLC‐like morphology. When Wnt signaling was activated in the flattened osteoblasts, they again acquired a cuboidal shape. Cuboidal and reactivated osteoblasts had many blebs on their cell membranes.
    Osteoblast Data, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44474 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+data/Imaris/pmc09664540-52-15-19
    Average 99 stars, based on 44474 article reviews
    osteoblast data - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Spatiotemporal Analysis of Osteoblast Morphology and Wnt Signal‐Induced Osteoblast Reactivation during Bone Modeling in Vitro"

    Article Title: Spatiotemporal Analysis of Osteoblast Morphology and Wnt Signal‐Induced Osteoblast Reactivation during Bone Modeling in Vitro

    Journal: JBMR Plus

    doi: 10.1002/jbm4.10689

    Schematic of osteoblast morphology and behavior during bone nodule formation. Osteoblasts had a crowded cuboidal morphology in the early stages of bone formation; some were embedded in the bone matrix and became osteocytes, and the remaining osteoblasts acquired a flattened BLC‐like morphology. When Wnt signaling was activated in the flattened osteoblasts, they again acquired a cuboidal shape. Cuboidal and reactivated osteoblasts had many blebs on their cell membranes.
    Figure Legend Snippet: Schematic of osteoblast morphology and behavior during bone nodule formation. Osteoblasts had a crowded cuboidal morphology in the early stages of bone formation; some were embedded in the bone matrix and became osteocytes, and the remaining osteoblasts acquired a flattened BLC‐like morphology. When Wnt signaling was activated in the flattened osteoblasts, they again acquired a cuboidal shape. Cuboidal and reactivated osteoblasts had many blebs on their cell membranes.

    Techniques Used:

    Cuboidal osteoblasts had many blebs. ( A ) Representative images of Day 10 osteoblasts after staining with phalloidin and 4,6‐diamidino‐2‐phenylindole. ( B ) Bleb formation observed in osteoblast membranes (yellow arrowhead indicates bleb). ( C ) Time‐lapse images of cuboidal osteoblasts and images obtained by subtracting minimum intensity from acquired images and changing color of luts. ( D ) Representative image cropped from ( C ). ( E ) Mean intensity of bleb regions. ( F ) Histogram of blebbing duration ( N = 82). ( G ) Histogram of maximum area of observed blebbing areas ( N = 123). Scale bar: 25 μm.
    Figure Legend Snippet: Cuboidal osteoblasts had many blebs. ( A ) Representative images of Day 10 osteoblasts after staining with phalloidin and 4,6‐diamidino‐2‐phenylindole. ( B ) Bleb formation observed in osteoblast membranes (yellow arrowhead indicates bleb). ( C ) Time‐lapse images of cuboidal osteoblasts and images obtained by subtracting minimum intensity from acquired images and changing color of luts. ( D ) Representative image cropped from ( C ). ( E ) Mean intensity of bleb regions. ( F ) Histogram of blebbing duration ( N = 82). ( G ) Histogram of maximum area of observed blebbing areas ( N = 123). Scale bar: 25 μm.

    Techniques Used: Staining

    Blebs have polarity, and cuboidal osteoblasts have high motility. ( A ) Representative images of time lapse in four dimensions and images extracted from frame‐by‐frame differences and their merged images. ( B ) Polar plots of distribution of bleb in xy , zy , and zx planes. ( C ) Violin plots of speed of cuboidal osteoblasts on bone matrix and adherent osteoblasts on dish, paired t ‐test. Scale bar: 25 μm.
    Figure Legend Snippet: Blebs have polarity, and cuboidal osteoblasts have high motility. ( A ) Representative images of time lapse in four dimensions and images extracted from frame‐by‐frame differences and their merged images. ( B ) Polar plots of distribution of bleb in xy , zy , and zx planes. ( C ) Violin plots of speed of cuboidal osteoblasts on bone matrix and adherent osteoblasts on dish, paired t ‐test. Scale bar: 25 μm.

    Techniques Used:

    Time‐lapse observation of changes in bone nodules and osteoblast morphology induced by BIO. ( A ) Representative images of osteoblast morphological changes from 0 to 55 hours after BIO administration. ( B ) Cropped image from ( A ) of osteoblasts that acquired a cuboidal shape after BIO administration (yellow arrows indicate blebs). ( C ) Schematic of experiment to analyze effects of BIO administration in long term. ( D ) Representative images of osteoblast morphological changes in BIO and vehicle groups (yellow arrows indicate osteoblasts that acquired a cuboidal morphology). ( E ) Line plots with error bars of SHG increase by BIO administration. ( F ) Changes in IN/ON osteoblast morphology over time following BIO administration. ( G ) Binarization of SHG and osteoblasts by IMARIS for maximum intensity projection. ( F ) SHG/EGFP ratio changes over time. N = 20 from five independent experiments. AU, arbitrary unit. Two‐sample t ‐test ( E , F , H ). Data shown as means ± SE. Scale bar: 50 μm.
    Figure Legend Snippet: Time‐lapse observation of changes in bone nodules and osteoblast morphology induced by BIO. ( A ) Representative images of osteoblast morphological changes from 0 to 55 hours after BIO administration. ( B ) Cropped image from ( A ) of osteoblasts that acquired a cuboidal shape after BIO administration (yellow arrows indicate blebs). ( C ) Schematic of experiment to analyze effects of BIO administration in long term. ( D ) Representative images of osteoblast morphological changes in BIO and vehicle groups (yellow arrows indicate osteoblasts that acquired a cuboidal morphology). ( E ) Line plots with error bars of SHG increase by BIO administration. ( F ) Changes in IN/ON osteoblast morphology over time following BIO administration. ( G ) Binarization of SHG and osteoblasts by IMARIS for maximum intensity projection. ( F ) SHG/EGFP ratio changes over time. N = 20 from five independent experiments. AU, arbitrary unit. Two‐sample t ‐test ( E , F , H ). Data shown as means ± SE. Scale bar: 50 μm.

    Techniques Used:

    Related Articles

    Staining:

    Article Title: Spatiotemporal Analysis of Osteoblast Morphology and Wnt Signal‐Induced Osteoblast Reactivation during Bone Modeling in Vitro
    Article Snippet: Delaunay triangulation was performed using the Delaunay triangulation function in MATLAB R2022a (MathWorks, USA) from osteoblast data quantified by IMARIS version 8.3.1 (Oxford Instruments) to calculate the area of the triangles.



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    osteoblast data  (Oxford Instruments)
    99
    Oxford Instruments osteoblast data
    Schematic of <t>osteoblast</t> morphology and behavior during bone nodule formation. Osteoblasts had a crowded cuboidal morphology in the early stages of bone formation; some were embedded in the bone matrix and became osteocytes, and the remaining osteoblasts acquired a flattened BLC‐like morphology. When Wnt signaling was activated in the flattened osteoblasts, they again acquired a cuboidal shape. Cuboidal and reactivated osteoblasts had many blebs on their cell membranes.
    Osteoblast Data, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+data/Imaris/pmc09664540-52-15-19
    Average 99 stars, based on 1 article reviews
    osteoblast data - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic of osteoblast morphology and behavior during bone nodule formation. Osteoblasts had a crowded cuboidal morphology in the early stages of bone formation; some were embedded in the bone matrix and became osteocytes, and the remaining osteoblasts acquired a flattened BLC‐like morphology. When Wnt signaling was activated in the flattened osteoblasts, they again acquired a cuboidal shape. Cuboidal and reactivated osteoblasts had many blebs on their cell membranes.

    Journal: JBMR Plus

    Article Title: Spatiotemporal Analysis of Osteoblast Morphology and Wnt Signal‐Induced Osteoblast Reactivation during Bone Modeling in Vitro

    doi: 10.1002/jbm4.10689

    Figure Lengend Snippet: Schematic of osteoblast morphology and behavior during bone nodule formation. Osteoblasts had a crowded cuboidal morphology in the early stages of bone formation; some were embedded in the bone matrix and became osteocytes, and the remaining osteoblasts acquired a flattened BLC‐like morphology. When Wnt signaling was activated in the flattened osteoblasts, they again acquired a cuboidal shape. Cuboidal and reactivated osteoblasts had many blebs on their cell membranes.

    Article Snippet: Delaunay triangulation was performed using the Delaunay triangulation function in MATLAB R2022a (MathWorks, USA) from osteoblast data quantified by IMARIS version 8.3.1 (Oxford Instruments) to calculate the area of the triangles.

    Techniques:

    Cuboidal osteoblasts had many blebs. ( A ) Representative images of Day 10 osteoblasts after staining with phalloidin and 4,6‐diamidino‐2‐phenylindole. ( B ) Bleb formation observed in osteoblast membranes (yellow arrowhead indicates bleb). ( C ) Time‐lapse images of cuboidal osteoblasts and images obtained by subtracting minimum intensity from acquired images and changing color of luts. ( D ) Representative image cropped from ( C ). ( E ) Mean intensity of bleb regions. ( F ) Histogram of blebbing duration ( N = 82). ( G ) Histogram of maximum area of observed blebbing areas ( N = 123). Scale bar: 25 μm.

    Journal: JBMR Plus

    Article Title: Spatiotemporal Analysis of Osteoblast Morphology and Wnt Signal‐Induced Osteoblast Reactivation during Bone Modeling in Vitro

    doi: 10.1002/jbm4.10689

    Figure Lengend Snippet: Cuboidal osteoblasts had many blebs. ( A ) Representative images of Day 10 osteoblasts after staining with phalloidin and 4,6‐diamidino‐2‐phenylindole. ( B ) Bleb formation observed in osteoblast membranes (yellow arrowhead indicates bleb). ( C ) Time‐lapse images of cuboidal osteoblasts and images obtained by subtracting minimum intensity from acquired images and changing color of luts. ( D ) Representative image cropped from ( C ). ( E ) Mean intensity of bleb regions. ( F ) Histogram of blebbing duration ( N = 82). ( G ) Histogram of maximum area of observed blebbing areas ( N = 123). Scale bar: 25 μm.

    Article Snippet: Delaunay triangulation was performed using the Delaunay triangulation function in MATLAB R2022a (MathWorks, USA) from osteoblast data quantified by IMARIS version 8.3.1 (Oxford Instruments) to calculate the area of the triangles.

    Techniques: Staining

    Blebs have polarity, and cuboidal osteoblasts have high motility. ( A ) Representative images of time lapse in four dimensions and images extracted from frame‐by‐frame differences and their merged images. ( B ) Polar plots of distribution of bleb in xy , zy , and zx planes. ( C ) Violin plots of speed of cuboidal osteoblasts on bone matrix and adherent osteoblasts on dish, paired t ‐test. Scale bar: 25 μm.

    Journal: JBMR Plus

    Article Title: Spatiotemporal Analysis of Osteoblast Morphology and Wnt Signal‐Induced Osteoblast Reactivation during Bone Modeling in Vitro

    doi: 10.1002/jbm4.10689

    Figure Lengend Snippet: Blebs have polarity, and cuboidal osteoblasts have high motility. ( A ) Representative images of time lapse in four dimensions and images extracted from frame‐by‐frame differences and their merged images. ( B ) Polar plots of distribution of bleb in xy , zy , and zx planes. ( C ) Violin plots of speed of cuboidal osteoblasts on bone matrix and adherent osteoblasts on dish, paired t ‐test. Scale bar: 25 μm.

    Article Snippet: Delaunay triangulation was performed using the Delaunay triangulation function in MATLAB R2022a (MathWorks, USA) from osteoblast data quantified by IMARIS version 8.3.1 (Oxford Instruments) to calculate the area of the triangles.

    Techniques:

    Time‐lapse observation of changes in bone nodules and osteoblast morphology induced by BIO. ( A ) Representative images of osteoblast morphological changes from 0 to 55 hours after BIO administration. ( B ) Cropped image from ( A ) of osteoblasts that acquired a cuboidal shape after BIO administration (yellow arrows indicate blebs). ( C ) Schematic of experiment to analyze effects of BIO administration in long term. ( D ) Representative images of osteoblast morphological changes in BIO and vehicle groups (yellow arrows indicate osteoblasts that acquired a cuboidal morphology). ( E ) Line plots with error bars of SHG increase by BIO administration. ( F ) Changes in IN/ON osteoblast morphology over time following BIO administration. ( G ) Binarization of SHG and osteoblasts by IMARIS for maximum intensity projection. ( F ) SHG/EGFP ratio changes over time. N = 20 from five independent experiments. AU, arbitrary unit. Two‐sample t ‐test ( E , F , H ). Data shown as means ± SE. Scale bar: 50 μm.

    Journal: JBMR Plus

    Article Title: Spatiotemporal Analysis of Osteoblast Morphology and Wnt Signal‐Induced Osteoblast Reactivation during Bone Modeling in Vitro

    doi: 10.1002/jbm4.10689

    Figure Lengend Snippet: Time‐lapse observation of changes in bone nodules and osteoblast morphology induced by BIO. ( A ) Representative images of osteoblast morphological changes from 0 to 55 hours after BIO administration. ( B ) Cropped image from ( A ) of osteoblasts that acquired a cuboidal shape after BIO administration (yellow arrows indicate blebs). ( C ) Schematic of experiment to analyze effects of BIO administration in long term. ( D ) Representative images of osteoblast morphological changes in BIO and vehicle groups (yellow arrows indicate osteoblasts that acquired a cuboidal morphology). ( E ) Line plots with error bars of SHG increase by BIO administration. ( F ) Changes in IN/ON osteoblast morphology over time following BIO administration. ( G ) Binarization of SHG and osteoblasts by IMARIS for maximum intensity projection. ( F ) SHG/EGFP ratio changes over time. N = 20 from five independent experiments. AU, arbitrary unit. Two‐sample t ‐test ( E , F , H ). Data shown as means ± SE. Scale bar: 50 μm.

    Article Snippet: Delaunay triangulation was performed using the Delaunay triangulation function in MATLAB R2022a (MathWorks, USA) from osteoblast data quantified by IMARIS version 8.3.1 (Oxford Instruments) to calculate the area of the triangles.

    Techniques: